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In a PCR reaction, the strands of DNA are first separated by |
heating |
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When is PCR particularly applicable? |
when there are small quantities of DNA to analyze |
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What is the correct sequence of events that occur in a PCR reaction? |
separation of DNA strands; addition of primers; use of DNA polymerase to produce second strand of DNA |
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DNA polymerase is a heat-sensitive enzyme. What is one thing that would need to be considered concerning the activity of this enzyme in PCR when the temperature is heated during each cycle to separate the DNA strands? |
that the DNA polymerase could be denature |
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Why are chromosomes cut into fragments for sequencing? |
A DNA sequencing reaction can only accurately determine about 500 bases of DNA. |
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A BAC library is "bacterial artificial chromosome." |
a collection of human DNA fragments carried on chromosomes in bacterial cells. |
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What is the major difference between the strategies of map-based sequencing and shotgun sequencing? |
In shotgun sequencing, DNA fragments are sequenced before their correct order is known. |
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Genes in the human genome sequence can be identified in all of the following ways EXCEPT |
matching genome sequences to proteins. |
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Which of the following build(s) new strands of DNA? |
DNA polymerases |
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Which statement about DNA replication is CORRECT? |
The leading strand is built continuously, and the lagging strand is built in pieces. |
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During DNA replication, which nucleotide will bind to an A nucleotide in the parental DNA? |
T |
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The molecule that seals the gaps between the pieces of DNA in the lagging strand is |
DNA ligase |
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Which statement about DNA replication is FALSE? |
DNA ligase adds nucleotides to the lagging strand. |
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What must happen before a cell can begin mitosis? |
The chromosomes must be duplicated. |
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The centrosomes move away from each other and the nuclear envelope breaks up during which phase of mitosis? |
Prophase |
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The chromosomes line up in the center of the cell during which phase of mitosis? |
Metaphase |
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The sister chromatids separate and begin moving toward opposite poles of the cell during which phase of mitosis? |
Anaphase |
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The chromosomes arrive at the poles and nuclear envelopes form during which phase of mitosis? |
Telophase |
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At the end of the mitotic (M) phase, the cytoplasm divides in a process called _________________. |
cytokinesis |
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