|
Which of the following describes the effect of boiling and freezing? |
Boiling denatured the enzyme, but freezing had no effect. |
|
What does a strong IKI result indicate? |
Substrate is present. |
|
The presence of reducing sugars _______. |
is detected using Benedict’s reagent |
|
Which tubes demonstrated amylase activity with a small amount of reducing sugar produced? |
tubes 7 and 8 |
|
Enzymes are _______. |
made of protein and are catalysts |
|
The substance that the enzyme acts on is called its _______. |
substrate |
|
Amylase is secreted by _______. |
both the salivary glands and the pancreas |
|
Maltose is a _______. |
disaccharide |
|
The purpose of this activity was to look at the effect of _______. |
enzyme/substrate specificity |
|
Which of the following enzyme/substrate combinations is matched INCORRECTLY? |
All are matched correctly. |
|
The cellulase in this experiment came from _______. |
bacteria |
|
What did the results of tube 3 demonstrate? |
Amylase does not digest cellulose. |
|
When using the Benedict’s assay, which color would indicate the most reducing sugar present? |
reddish brown |
|
IKI is used to test for the presence of _______. |
starch and cellulose |
|
Substrates are held in the active site by _______. |
hydrogen and ionic bonds |
|
What is the difference between cellulose and starch? |
They are linked differently. |
|
What occurred when pepsin was boiled? |
It was inactivated. |
|
Pepsin is _______. |
a peptidase, an enzyme that digests protein and a protein that digests protein |
|
Which test tubes were used to determine the optimal pH for pepsin activity? |
tubes 2, 5, and 6 |
|
The optimal pH for pepsin activity is _______. |
2 |
|
The building blocks for peptides are _______. |
amino acids |
|
Pepsin is secreted by _______. |
chief cells |
|
Pepsin is classified as _______. |
an enzyme and a peptidase |
|
When BAPNA is hydrolyzed, it _______. |
changes from colorless to yellow |
|
Which test tubes were used to determine the optimal pH for lipase activity? |
tubes 1, 5, and 6 |
|
Which test tube had the highest lipase activity? |
tube 1 |
|
Why is gastric lipase NOT as active as pancreatic lipase? |
The pH of the stomach is too low and no bile is present in the stomach. |
|
The substrate of lipase is _______. |
triglyceride |
|
Triglycerides _______. |
All of the above are true. |
|
Adding bile to triglycerides _______. |
increases the surface area of the triglycerides |
|
Which of following does NOT secrete a lipase? |
the small intestine |
|
Prior to lipase digestion of triglycerides, the solution would be _______. |
more basic |
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