|
What are the functions of endospores in bacteria? Lab Report Question |
Endospores are resting stages that allow bacteria to survive conditions unfavorable for growth. |
|
What external structure on the endospore acts as a protective barrieR? What is its composition? Lab Report Question |
Endospores have a protective barrier on their exterior called an exosporium that is composed of protein. |
|
Compared to a vegetative cell, how much less water is present in an endospore? Lab Report Question |
Endospores are very dehydrated structures that are not actively metabolizing. The water content is reduced to 10-30% of the vegetative cell. So, there is 70-90% less water in the endospore. |
|
What is the mordant in the spore stain? Lab Report Question |
heat; it cuases the endospore to expand allowing the stain to penetrate the structure. |
|
What is the stimulus for endospore production in bacteria? Lab Report Question |
decrease of nutrients in the environment |
|
Destroying Endospores |
Endospores must be exposed to 15-20 minutes of steam pressure at a temperature of 121 degrees celsius. Conditions produced in an autoclave. |
|
What is the color of endospores after gram staining? |
After gram staining, the endospore is colorless. After the endospore stain, it is green |
|
What is the secondary stain in the spore stain? |
Safranin is the secondary stain in the endospore stain and is necessary to stain the vegetative cell. |
|
Are bacterial endospores reproductive structures? Explain why or why not. |
no they are dormant structures that can grow through the process of germination if nutrients become available again. |
|
What is the color of the vegetative cell after the spore stain? |
Vegatative cell–red |
|
What is the genera of bacteria that produces endospores |
Clostridium, Bacillus |
|
Give three examples of diseases caused by an endospore-forming bacterium and the name of the specific bacterial agent involved. |
… |
Share This
Flashcard
