Microbiology Chapter 5 Flashcards

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One of the first chemicals used by Lister to prevent surgical sepsis was
a) alcohol
b) iodine
c) carbolic acid
d) mercury

c) carbolic acid

the process of killing or removing all the microorganisms in or on a meterial is termed
a) sterilization
b) disinfection
c) sanitation
d) antisepsis

a) sterilizationn

A sterile item is free of
a) microbes
b) endospores
c) viruses
d) prions
e) A, B, and C

e) A, B, and C

suffix used to describe a killing action would be
a) -static
b) -cidal
c) -cilllin
d) -tion

b) – cidal

Pasteurization
a) use of heat to sterilize food products
b) use of heat to reduce pathogenic/spoilage bacteria to a safe level
c) process which uses intense cold to kill microorganisms on food
d) process which uses short burst of radiation to kill microorganisms on food

b) use of heat to reduce pathogenic/spoilage bacteria to a safe level

plain soap is very effective in controlling spread of microorganism because it is
a) bacteriostatic
b) very effective at the mechanical removal of microorganism
c) virucidal
d) bactericidal

b) very effective at the mechanical removal of microorganism

Nosocmial infections
a) are acquired at various social events
b) are acquired while in the hospital
c) occur because of a susceptible population and presence of disease causing organisms
d) are acquired at sporting events
e) B and C

e) B and C

to reduce or eliminate disease/spoilage causing organisms, food is often subjected to
a) heat
b) chemical additives
c) radiation
d) cold
e) all the above are correct

e) all the above are correct

which of the following organisms are resistant to destruction by typical control methods
a) endospores of Bacillus and Clostridium
b) Pseudomonas
c) naked viruses
d) Mycobacterium spp
e) all of the choices are correct

e) all of the choices are correct

a common environmental organism that may even grow in certain chemical disinfectants is
a) Escherichia coli
b) Streptocuccus pnemoniae
c) Pseudomonas aeruginosa
d) enveloped virus

c) Pseudomonas aeruginosa

upon treatment with heat or chemicals, bacteria will
a) all die immediately
b) die at a constant proportion
c) die at an exponential rate
d) die at a geometric rate

b) Die at a constant proportion

microbial death rates may be affected by
a) pH
b) temperature
c) the presence of organics
d) all of the choices are correct

d) all of the choices are correct

In order to speed up the sterilization process, which of the following would be useful
a) drying the material
b) washing/mechanical removal of bacteria/organic matter
c) addition of organics
d) nothing
e) B and C

b) washing/mechanical removal of bacteria/organic matter

moist heat kills microorganisms by
a) irreversible coagulation of proteins
b) destruction of carbohydrates in the cell wall
c) denaturation of nucleic acids
d) dissolving the capsule

a) irreversible coagulation of proteins

boiling is not reliable for sterilization because
a) heat sensitive instruments may be destroyed
b) heat resistant endospores are unaffected
c) water boils at a higher temperature at lower altitudes
d) viruses are more sensitive to heat than bacteria

b) heat resistant endospores are unaffected

typical conditions used for sterilization are
a) 100 degrees C for 10 minutes
b) 121 degrees C at 15 psi for 15 minutes
c) 80 degrees C for 15 minutes
d) 72 degrees C for 15 seconds

b) 121 degrees C at 15 psi for 15 minutes

which of the following is not a sterilization method
a) hot air oven
b) autoclave
c) pasteurization
d) diltration

c) pasteurization

commercial canning processes
a) geared to destroy Clostridium botulinum spores
b) kill all endospores
c) are especially needed on low acid foods
d) are 12D processes
e) A, C, and D

e) A, C, and D

a common application of dry heat in the laboratory is to
a) prepare specimens for study
b) sterilize media
c) sterilize plastic
d) sterilize the inoculating loop

d) sterilize the inoculating loop

ultraviolet radiation at the bacterial wavelength destroy bacteria by
a) destroying endospores
b) damaging nucleic acid
c) preventing spore formation
d) denaturing proteins

b) damaging nucleic acid

microwaves do not kill organisms directly buy kill by
a) the heat they generate in a product
b) generating free radicals
c) genreating toxins
d) creating thymine dimers

a) the heat they generate in a product

chemical germicides
a) may react irreversibly with proteins/enzymes
b) may react with cytoplasmic membranes or viral envelopes
c) may be disinfecting or even sterilizing
d) are sensitive to dilution factor, time of contact, and temperature of use
e) all of the choices are correct

e) all of the choices are correct

the most important function of nitrites in processed foods is to
a) prevent browning
b) inhibit the germination of Clostridium botulinum endospores
c) prevent carcinogen formation
d) make the food more acidic

b) inhibit the germination of Clostridium botulinum endospores

Which of the foolowing is (are) considered when selecting a germicidal chemical
a) toxicity
b) cost
c) compatibility with the material being treated
d) environmental impact
e) all of the choices are correct

e) all of the choices are correct

Prions and viroids are easily destroyed by common sterilization procedures
a) true
b) false

b) false

the more bacteria one starts with, the longer it will take to kill them all
a) true
b) false

a) true

Heat treatment is an effective method for sterilization or disinfection of all materials
a) true
b) false

b) false

boiling is very effective at removing most common waterborne pathogens
a) true
b) false

a) true

Pasteurization results in the sterilization of food products
a) true
b) false

b) false

generally, heavy metals, except silver, have been proven to be too toxic for use on human tissue and are no longer used medically
a) true
b) false

a) true

Hydrogen peroxide may be used as a sterilant on living tissues
a) true
b) false

b) false

cold and freezing are very effective in killiing bacteria
a) true
b) false

b) false

which of the following is not affected by an antimicrobial procedure
a) cell membrane
b) size of the microbe
c) proteins, such as enzymes
d) DNA

b) size of microbe

which of the following concerns is the mnost important when choosing an antimicrobial procedure
a) how much time it will take
b) the organism trying to eliminate
c) materials trying to remove organism from
d) the people who will come into contact with the residue from the chemical

b) organism trying to eliminate

lysol is an acceptable aseptic chemical
a) true
b) false

b) false

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