Micro Ch. 10

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1) Which of the following statements about archaea is FALSE?
A) They are prokaryotes.
B) They lack peptidoglycan in their cell walls.
C) Some are thermoacidophiles; others are extreme halophiles.
D) They evolved before bacteria.
E) Some produce methane from carbon dioxide and hydrogen.

D) They evolved before bacteria.

2) If two organisms have similar rRNA sequences, you can conclude that they
A) live in the same place.
B) evolved from a common ancestor.
C) will have different G-C ratios.
D) will both ferment lactose.
E) mated with each other.

B) evolved from a common ancestor.

3) Which of the following statements about members of the Kingdom Plantae is FALSE?
A) They are multicellular.
B) They are composed of eukaryotic cells.
C) They undergo photosynthesis.
D) They use organic carbon sources.
E) They synthesize organic molecules.

D) They use organic carbon sources.

4) Which of the following statements about the members of the Kingdom Animalia is FALSE?
A) They are multicellular.
B) They are composed of eukaryotic cells.
C) They undergo photosynthesis.
D) They ingest nutrients through a mouth.
E) They are heterotrophs.

C) They undergo photosynthesis.

5) A genus can best be defined as
A) a taxon composed of families.
B) a taxon composed of one or more species and a classification level lying below family.
C) a taxon belonging to a species.
D) a taxon comprised of classes.
E) the most specific taxon.

B) a taxon composed of one or more species and a classification level lying below family.

6) A bacterial species differs from a species of eukaryotic organisms in that a bacterial species
A) does not breed with other species.
B) has a limited geographical distribution.
C) can be distinguished from other bacterial species.
D) is a population of cells with similar characteristics.
E) breeds with its own species.

D) is a population of cells with similar characteristics.

7) Which of the following is the best evidence for a three-domain system?
A) Nucleotide sequences in ribosomal RNA vary between all three domains.
B) There are three distinctly different sets of metabolic reactions.
C) There are three distinctly different Gram reactions.
D) Some bacteria live in extreme environments.
E) There are three distinctly different types of nuclei.

B) There are three distinctly different sets of metabolic reactions.

8) A biochemical test, in the microbiology world, is used to determine
A) staining characteristics.
B) amino acid sequences of enzymes.
C) nucleic acid-base composition of DNA sequences.
D) capability of a microbe to perform a specific enzymatic activity.
E) All of the answers are correct.

D) capability of a microbe to perform a specific enzymatic activity.

9) The phylogenetic classification of bacteria is based on
A) cell morphology.
B) Gram reaction.
C) rRNA sequences.
D) habitat.
E) diseases.

C) rRNA sequences.

10) Which of the following statements is a reason for NOT classifying viruses in one of the three
domains of life (Bacteria, Archaea, Eukarya) rather than in a fourth separate domain?
A) Some viruses can incorporate their genome into a host’s genome.
B) Viruses direct anabolic pathways of host cells.
C) Viruses are obligate parasites.
D) Viruses are not composed of cells.
E) All of the answers are correct.

D) Viruses are not composed of cells.

11) Which of the following provides taxonomic information that includes the information obtained in the others listed?
A) nucleic acid hybridization
B) nucleic acid-base composition
C) amino acid sequencing
D) biochemical tests
E) cladogram

E) cladogram

Figure 10.1

12) In Figure 10.1, species "c." is most closely related to which of the following?
A) a
B) b
C) c
D) d
E) e

D) d

13) In Figure 10.1, the closest ancestor for both species "a." and species "b." would be which of the following?
A) 1
B) 2
C) 3
D) 4
E) 5

E) 5

14) Protists are a diverse group of organisms that are similar in
A) rRNA sequences.
B) metabolic type.
C) motility.
D) ecology.
E) None of the answers is correct.

E) None of the answers is correct.

15) In the scientific name Enterobacter aerogenes, Enterobacter is the
A) specific epithet.
B) genus.
C) family.
D) order.
E) kingdom.

B) genus.

16) The arrangement of organisms into taxa
A) shows degrees of relatedness between organisms.
B) shows relationships to common ancestors.
C) was designed by Charles Darwin.
D) is arbitrary.
E) is based on evolution.

A) shows degrees of relatedness between organisms.

17) Bacteria and Archaea are similar in which of the following?
A) peptidoglycan cell walls
B) methionine as the start signal for protein synthesis
C) sensitivity to antibiotics
D) considered prokaryotic cells
E) plasma membrane ester linkage

E) plasma membrane ester linkage

18) Which of the following best defines a strain in microbiological terms?
A) a pure culture that is not totally identical to other cultures of the same species
B) a group of organisms with a limited geographical distribution
C) a mixed population of cells,genetically distinct, but with highly similar phenotypic characteristics
D) groups of cells all derived from and identical to a single parent species
E) same as a species

C) a mixed population of cells,genetically distinct, but with highly similar phenotypic characteristics

19) Serological testing is based on the fact that
A) all bacteria have the same antigens.
B) antibodies react specifically with an antigen.
C) the human body makes antibodies against bacteria.
D) antibodies cause the formation of antigens.
E) bacteria clump together when mixed with any antibodies.

B) antibodies react specifically with an antigen.

20) Phage typing is based on the fact that
A) bacteria are destroyed by viruses.
B) viruses cause disease.
C) bacterial viruses attack only members of a specific species.
D) Staphylococcus causes infections.
E) phages and bacteria are related.

A) bacteria are destroyed by viruses.

21) Which of the following criteria is most useful in determining whether two organisms are related?
A) Both ferment lactose.
B) Both are gram-positive.
C) Both are motile.
D) Both are aerobic.
E) Each answer is equally important.

E) Each answer is equally important.

22) A clone is
A) genetically identical cells derived from a single cell.
B) a genetically engineered cell.
C) a taxon composed of species.
D) a mound of cells on an agar medium.
E) None of the answers is correct.

A) genetically identical cells derived from a single cell.

Table 10.1

1. 9+2 flagella
2. Nucleus
3. Plasma membrane
4. Peptidoglycan
5. Mitochondrion
6. Fimbriae

23) In Table 10.1, which features are found in nearly all eukarya?
A) 2, 3, 5
B) 1, 4, 6
C) 3, 5
D) 2, 3
E) 1, 2, 5

A) 2, 3, 5

Table 10.1

1. 9+2 flagella
2. Nucleus
3. Plasma membrane
4. Peptidoglycan
5. Mitochondrion
6. Fimbriae

24) In Table 10.1, which feature(s) is (are) found only in prokaryotes?
A) 1, 2, 3
B) 4, 6
C) 2
D) 1
E) 2, 4, 5

B) 4, 6

25) Into which group would you place a multicellular heterotroph with chitin cell walls?
A) Animalia
B) Archaea
C) Bacteria
D) Fungi
E) Plantae

D) Fungi

26) Figure 10.3

This figure shows the results of a gel electrophoresis separation of restriction fragments of the DNA of different organisms.

In Figure 10.3, which two are most closely related?
A) 1 and 3
B) 2 and 4
C) 3 and 5
D) 2 and 5
E) 4 and 5

A) 1 and 3

27) Into which group would you place a photosynthetic cell that lacks a nucleus?
A) Animalia
B) Bacteria
C) Fungi
D) Plantae
E) Protista

E) Protista

28) You discovered a unicellular organism that lacks a nucleus and peptidoglycan. You suspect the organism is in the group
A) Animalia.
B) Archaea.
C) Bacteria.
D) Fungi.
E) Plantae.

B) Archaea.

29) Into which group would you place a unicellular organism that has 70S ribosomes and a peptidoglycan cell wall?
A) Animalia
B) Bacteria
C) Fungi
D) Plantae
E) Protist

B) Bacteria

Table 10.2

30) Use the dichotomous key in Table 10.2 to identify a gram-negative cell that ferments lactose and uses citric acid as its sole carbon source.
A) Citrobacter
B) Escherichia
C) Lactobacillus
D) Pseudomonas
E) Staphylococcus

A) Citrobacter

31) Use the dichotomous key in Table 10.2 to identify a gram-negative coccus.
A) Neisseria
B) Pseudomonas
C) Staphylococcus
D) Streptococcus
E) Micrococcus

A) Neisseria

1) The cell membranes of all three domains are composed of straight carbon chains attached to
glycerol by ester linkage.

False

2) Chloroplasts possess circular DNA and reproduce by binary fission.

True

3) As molecular techniques improve, scientists are finding it necessary to reclassify some genera.

True

4) The highest level in the current taxonomic hierarchy is "Kingdom."

False

5) Pure cultures of the same species are not always identical in all ways.

True

6) At this point in time, scientists believe the vast majority of the domain Bacteria have been discovered.

False

7) Once a culture is purified, the next logical step in the process of identifying bacteria is biochemical testing.

False

8) Serological testing is used for screening bacterial isolates for similarities.

True

9) Western blotting is an immunological test used to identify the antibodies in the serum of an individual suspected of having Lyme disease.

False

10) When building a cladogram, the length of the horizontal branch is based on the calculated percent similarity between organisms.

True

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