In eukaryotic BER, long patch repair replaces _______ nucleotides, and a short patch repair replaces ______ nucleotides?
500-700; 5 |
2-10; 1 |
What step is NOT part of excision repair? Excision by exonuclease |
Dam methylation |
Which statement is TRUE in regard to eukaryotic transcription-linked nucleotide excision repair NER? Global genome repair pathway does not share any common steps with transcription-linked nucleotide excision repair. The large subunit of RNA polymerase is degraded; TFIIH remains and recruits XP proteins to repair DNA damage. In eukaryotic nucleotide excision repair DNA polymerase V synthesized DNA after damage is removed. Yeast Rad4 recognizes damaged DNA and this process involves flipping out thymine dimers. |
The large subunit of RNA polymerase is degraded; TFIIH remains and recruits XP proteins to repair DNA damage. |
In the uvr excision repair system in E. coli, which enzyme recruits uvrC? UvrD |
… |
In the uvr excision repair system in E. coli, which steps DO NOT require hydrolysis of ATP? UvrA release from damaged DNA UvrA recognition of damaged nucleotide UvrD helicase DNA unwinding UvrBC nicking on either side of damaged nucleotide |
… |
In the uvr excision repair system of E. coli, long patch repair replaces _______ nucleotides, and a short patch repair replaces ______ nucleotides? 00-700; 5 2-10; 1 1500-9000; 12 700-900; 24 |
1500-9000; 12 |
Which of the following is NOT the activity of Mfd protein? It recruits the uvrAB proteins and directs repair to the damaged template strand. It displaces RNA polymerase. It binds to stalled RNA polymerase. It degrades RNA polymerase |
2-10; 1 |
Which statement is FALSE in regard to eukaryotic base excision repair BER? The nature of base removal in BER determines which polymerase is recruited to the DNA by AP1 protein, either delta epsilon or beta. Base removal triggers the removal and replacement of a stretch of polynucleotide, using either long patch or short path repair. In BER, polymerase delta and epsilon replaces long stretch of nucleotides, which is 1500-9000 bases. BER starts by directly removing a damaged base from DNA. |
In BER, polymerase delta and epsilon replaces long stretch of nucleotides, which is 1500-9000 bases. |
What is the enzyme in bacteria that directly photoreactivates pyrimidine dimers? AP1 endonuclease MutS, L Photolyase phr FEN1 endonuclease |
Photolyase phr |
In base excision repair, the uracil glycosylase enzyme corrects DNA damage by biased replacement of ________ to ______? Depurinated nucleotide; insertion of nucleotide U-G; C-G Methyl-G; unmethylated G T-T dimer; A-A |
U-G; C-G |
Which type of DNA damage does NOT result in stopped replication or stopped transcription? Depurination Base alkylation; DNA nicking Mismatch; deamination |
Mismatch; deamination |
In the uvr excision repair system in E. coli, which enzyme unwinds damaged DNA? UvrD UvrC UvrB UvrA |
… |
Which type of DNA damage does NOT result in stopped replication or stopped transcription? Thymine dimers Depurination Base alkylation; DNA nicking Mismatch; deamination |
… |
What protein is uniquely linked to transcription and DNA repair in eukaryotes? TFIIH helicases XPB and XPD |
TFIIH helicases XPB and XPD |
In the uvr excision repair system in E. coli, which enzyme routinely synthesizes DNA to replace the excised strand? DNA polymerase V |
DNA polymerase I |
What protein is uniquely linked to transcription and DNA repair in E. coli? Reverse transcriptase |
… |
HQ-17 Homework-Repair Module A
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