1. In the process of translation, _____. |
Proteins are synthesized |
2. In the process of transcription, _____. |
RNA is synthesized |
3. According to the central dogma, what molecule should go in the blank? DNA → _____ → Proteins |
mRNA |
4. Codons are part of the molecular structure of _____. |
mRNA |
5. Once researchers identified DNA as the unit of inheritance, they asked how information was transferred from the DNA in the nucleus to the site of protein synthesis in the cytoplasm. What is the mechanism of information transfer in eukarotes? |
Messenger RNA is transcribed from a single gene and transfers information from the DNA in the nucleus to the cytoplasm, where protein synthesis takes place. |
6. Translation requires _____. |
mRNA, tRNA and rRNA |
7. A nonsense mutation in a gene _____. |
introduces a premature stop codon into the mRNA |
8. The most commonly occurring mutation in people with cystic fibrosis is a deletion of a single codon. This results in _____. |
a polypeptide missing an amino acid |
9. How might a single base substitution in the sequence of a gene affect the amino acid sequence of a protein encoded by the gene, and why? (1pt) |
It might cause a different protein to be expressed due to the change of the amino acid sequence, causing the phenotype of the organism to change. One letter change can cause a completely different protein to be produced. |
10. Which of the following types of mutation, resulting in an error in the mRNA just after the AUG start of translation, is likely to have the most serious effect on the polypeptide product? |
A deletion of two nucleotides |
11. What does it mean when we say that the genetic code is redundant and unambiguous? (2pts) |
It means that all amino acids except methionene and tryptophan are coded by more than one codon and that a single codon never codes for more than one amino acid. |
12. List the 3 distinct characteristics of the genetic code and explain why they are important. (3pts) |
1) It is non-overlapping: once the ribosome locks onto the first codon, it then reads each separate codon one after another 2) The code is nearly universal: all codons specify the same amino acids in all organisms 3) The code is conservative: when several codons specify the same amino acid, the first two bases in those codons are most always identical |
13. In an experimental situation, a student researcher inserts an mRNA molecule into a eukaryotic cell after she has removed its cap and poly-A tail. Which of the following would you expect her to find? |
The molecule is digested by enzymes because it is not protected at the 5′ end |
14. Which molecule or reaction supplies the energy for polymerization of nucleotides in the process of transcription? |
The phosphate bonds in the nucleotide triphosphates that serve as substrates |
15. What ensures that the correct amino acid is added during translation? |
The anticodon of a properly formed aminoacyl tRNA |
16. A primary transcript in the nucleus of a eukaryotic cell is _____ the functional mRNA, while a primary transcript in a prokaryotic cell is _____ the functional mRNA. |
Larger than; the same size as |
17. Which of the following contradicts the one-gene, one-enzyme hypothesis? |
A single antibody gene can code for different related proteins, depending on the splicing that takes place post transcriptionally |
18. How does termination of translation take place? |
A stop codon is reached |
19. Codons are three-base sequences that specify the addition of a single amino acid. How do eukaryotic codons and prokaryotic codons compare? |
Codons are a nearly universal language among all organisms |
20. Which of the following is the first event to take place in translation in eukaryotes? |
The small subunit of the ribosome recognizes and attaches to the cap of mRNA |
21. A particular triplet of bases in the template strand of DNA is 5′ AGT 3′. The corresponding codon for the mRNA transcribed is _____. |
UCA |
23. Which of the following levels of gene expression allows the most rapid response to environmental change? |
Post translational control |
24. The reason for differences in the sets of proteins expressed in a nerve and a pancreatic cell of the same individual is that nerve and pancreatic cells contain different _____. |
Sets of regulatory proteins |
25. In negative control, a gene is switched off when _____. |
a regulatory protein binds to DNA and shuts down transcription |
26. The trp repressor blocks transcription of the trp operon when the repressor _____. |
binds to tryptophan |
27. Tumor-suppressor genes _____. |
encode proteins that help prevent uncontrolled cell growth |
28. Altering patterns of gene expression in prokaryotes would most likely serve an organism’s survival by _____. |
allowing organisms to adjust to changes in environmental conditions |
29. E. coli can alter its production of amino acids based on the availability of those amino acids in the environment. The trp repressor blocks transcription of the trp operon for producing the amino acid tryptophan when the repressor _____. |
… |
30. Not long ago, it was believed that a count of the number of protein-coding genes would provide a count of the number of proteins produced in any given eukaryotic species. This is incorrect, largely due to the discovery of widespread _____. |
alternative splicing |
31. Steroid hormones produce their effects in cells by _____. |
binding to intracellular receptors and promoting transcription of specific genes |
32. Imagine that you have set up a genetic screen to identify E. coli mutants that cannot metabolize the amino acid tryptophan for energy. Beginning with a master plate containing many colonies, you prepare replica plates on medium with glucose or tryptophan as the only energy source. You would look for colonies that _____. |
can grow only on the plates with glucose |
34. Compare and contrast transcription and DNA replication (2 ways they are similar, and 2 ways they are different). (2pts) |
Similarities: Template-directed synthesis by polymerases in a 5′ to 3′ direction Differences: RNA polymerase doesn’t require a primer Sigma guides RNA polymerase to specific locations while transcription is happening |
Explain the ‘central dogma’ of biology |
DNA–transcription–>RNA–translation–>PROTEIN |
Identify different types of mutations and how they can affect gene expression |
Beneficial, Neutral, Deleterious |
Explain the three possible mechanisms of gene expression regulation in prokaryotes |
1) Transcriptional control: 2) Translational control: 3) Post-translational control: |
Compare and contrast negative and positive control |
Negative control causes transcription to stop due to repressor proteins; Positive control triggers transcriotion due to activator proteins and regulatory proteins. |
Transcription |
The process of copying hereditary information in DNA to RNA |
Translation |
The process of using the information in nucleic acid to synthesize proteins |
Missense mutation |
A point mutation that causes a change in the amino acid expression of the organism |
Silent mutation |
A point mutation that does not cause a change in the amino acid expression of the organism |
Frameshift mutation |
Addition or deletion of a codon that alters the meaning of all subsequent codons |
Nonsense mutation |
A point mutation that causes the expression of an amino acid to be changed to a stop codon |
Initiation of Translation |
Holoenzymes (RNA polymerase and sigma in bacteria; a combination of basal transcription factors) bind to promoters; DNA is separated and NTPs are added so that mRNA is polymerized and extended from the site of transcription. |
Elongation of Translation |
Prominent channels and grooves in the enzyme are filled |
Termination of Transcription |
RNA polymerase transcribes a DNA sequence that functions as a termination signal; a hairpin loop forms and causes the mRNA to separate from RNA polymerase |
Intron |
Portion of RNA that is spliced out because it doesn’t code for any information |
Exon |
Portion of RNA that is joined together after introns are spliced out; codes for necessary information |
RNA processing |
Happens in eukaryotic cell transcription; includes: 1) Addition of 5′ cap on mRNA 2) Splicing of introns 3) Addition of 3′ poly-A tail on mRNAs |
Translational control |
Allows the cell to quickly change which proteins are produced |
Post-translational control |
Folding: Sped up by "molecular chaperones" Chemical modifications: Chemical groups added in the Golgi and ER, alteration by enzymes |
Compare and contrast transcription and translation |
Transcription: -Synthesis of RNA -Uses genes as a template -Located in the nucleus Translation: -Synthesize proteins -Uses mRNA as a template -Located in the ribosomes |
tRNA |
It is the type of RNA that translates between mRNA and proteins. It has a specific hairpin loop structure, and at the end of one of the loops contains an anticodon that recognizes and decodes mRNA so that it can be made into proteins. |
rRNA |
The assembly plant where everything comes together to synthesize proteins from mRNA |
Given the template strand: 5’AGT3′ |
mRNA: 3’UCA5′ or 5’ACU3′ tRNA: 3’UGA5′ |
What does RNA polymerase do? |
It synthesizes mRNA by catalyzing the reaction that cleaves off 2 phosphates and forms a phosphodiester bond between the 3′ end of the mRNA chain and the new ribonucleoside monophosphate. |
Bio 115 unit 4
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