Among the microorganisms, various genomes can include |
E. All of the choices are correct. |
Eukaryotic chromosomes differ from prokaryotic chromosomes because only eukaryotes have |
E. All of the choices are correct. |
Which of the following is not true of an organism’s genotype? |
E. are the expressed traits governed by the genes |
The _____ is all of the genetic material of a cell. |
D. genome |
Each _____ is a specific segment of the DNA with the code for production of one functional product. |
C. gene |
The expression of genetic traits is the |
D. phenotype. |
The DNA of microorganisms is made up of subunits called |
C. nucleotides. |
Which is incorrect about purines? |
A. only found in DNA, not in RNA |
The antiparallel arrangement within DNA molecules refers to |
C. one helix strand that runs from the 5′ to 3′ direction and the other strand runs from the 3′ to 5′ direction. |
All of the following pertain to nitrogenous bases except |
B. guanine pairs with uracil. |
Each nucleotide is composed of |
A. one phosphate, one nitrogenous base, and one sugar. |
Base pairs in DNA are held together by _____ bonds. |
D. hydrogen |
The duplication of a cell’s DNA is called |
B. replication. |
Structural genes code for |
C. cellular proteins. |
During replication, each parent DNA strand serves as a _____ for synthesis of new DNA strands. |
B. template |
Semi-conservative replication refers to |
D. an original parent DNA strand and one newly synthesized DNA strand comprising a new DNA molecule. |
DNA polymerase III |
B. synthesizes new DNA only in the 5′ to 3′ direction. |
Okazaki fragments are attached to the growing end of the lagging strand by |
A. DNA ligases. |
The enzymes that can proofread replicating DNA, detect incorrect bases, excise them, and correctly replace them are |
B. DNA polymerases. |
The site where the old DNA strands separate and new DNA strands will be synthesized is called the |
E. replication fork. |
Helicase |
A. unzips DNA. |
DNA Polymerase I |
A. removes primers. |
DNA Polymerase III |
E. proofreads new DNA. |
A permanent, inheritable change in the genetic information is called |
C. mutation. |
Replication of DNA begins at a/an |
C. adenine-thymine rich area. |
A primer of ____ is needed at the origin of nucleotide addition. |
D. RNA |
Groups of three consecutive bases along the DNA of a gene have the code for one |
C. amino acid. |
The three base sequence on DNA is called a/an |
E. triplet. |
The RNA molecules that carry amino acids to the ribosomes during protein synthesis are called |
C. transfer RNA. |
This molecule is synthesized as a copy of a gene on the DNA template strand: |
B. messenger RNA |
RNA molecules differ from DNA molecules because only RNA |
E. All of the choices are correct. |
All of the following pertain to transcription except |
A. occurs on a ribosome in the cytoplasm. |
The nontranscribed region of DNA to which RNA polymerase binds to initiate transcription is called the |
A. promoter. |
Which of the following is incorrect about transfer RNA? |
E. initiator tRNA in bacteria carries tryptophan |
If a codon for alanine is GCA, then the anticodon is |
D. CGU. |
The following pertain to ribosomes during protein synthesis except |
A. participate in both transcription and translation. |
Which of the following is incorrect about termination codons? |
C. include AUG |
Which of the following is not associated with a prokaryotic ribosome? A. is a 70s |
D. small unit provides the enzymes for making peptide bonds |
A sequence of bases on a gene that does not code for protein is called a/an |
E. intron. |
Split genes |
D. use spliceosomes to excise introns and then join exons. |
Which is incorrect about inducible operons? |
A. have genes turned off by a buildup of end product |
The operon segment composed of the gene that codes for a protein capable of repressing the operon is called the |
C. regulator. |
Synthesis of an inducible enzyme requires |
B. substrate bound to repressor. |
Full induction of the lactose operon requires |
C. lactose present without glucose. |
The lactose repressor |
C. is inactivated by binding lactose. |
Gene regulation can involve a protein repressor that blocks _____ from initiating transcription. |
C. RNA polymerase |
A mutation that changes a normal codon to a stop codon is called a |
E. nonsense mutation. |
The most serious type of mutation is a |
C. frame shift mutation. |
If the wild type DNA sequence reads THE CAT ATE THE BIG RAT, what type of mutation would change the sequence to THE CAT ATA ETH EBI GRA T? |
C. insertion |
If the wild type DNA sequence reads THE CAT ATE THE BIG RAT, what type of mutation would change the sequence to THE CAT ATE THE BAG RAT? |
A. missense |
If the wild type DNA sequence reads THE CAT ATE THE BIG RAT, what type of mutation would change the sequence to THE CAT ATE (stop)? |
B. nonsense |
A frame shift is caused by ______ mutations. |
D. deletion and insertion |
What type of mutation alters the base, but not the amino acid being coded for? |
A. silent |
The process in which mutations are removed and the correct bases added is called |
B. excision repair. |
Bacterial conjugation involves |
B. a donor cell with a plasmid that synthesizes a pilus. |
Which cell can transfer the most DNA? |
C. Hfr cell |
Which of the following is not a type of bacterial DNA recombination? |
C. mitosis |
Which of the following is not true of conjugation? |
D. transfers genes for a polysaccharide capsule |
Hfr transfer involves all of the following except |
B. plasmid gene transfer. |
The transfer of DNA fragments from a dead cell to a live, competent recipient cell that results in a changed recipient cell is |
C. transformation. |
The development of virulent, toxin-producing bacterial strains due to the presence of a temperate phage can occur in |
D. specialized transduction. |
A bacteriophage transfers DNA of the previous host to the current host. This is an example of |
B. generalized transduction. |
The jumping of a gene from one location to another is done by |
B. transposons. |
Which of the following is not true of transposons? |
E. are always part of plasmids |
The smallest unit of heredity is a chromosome. |
false |
DNA replication proceeds in one direction around the bacterial chromosome. |
false |
Transcription occurs in the nucleus or at the nucleoid. |
true |
In bacteria, initiator tRNA carries formyl methionine. |
true |
Excised introns form structures called lariats. |
true |
Lactose is a corepressor in the lac operon. |
false |
Repressible operons are normally turned on in the cell. |
true |
Induced mutations result from errors in DNA replication. |
false |
DNA photolyase, in the presence of visible light, can repair DNA damage due to ultraviolet radiation. |
true |
All mutations are not beneficial to the cell. |
false |
The various techniques by which scientists manipulate DNA in the lab are called |
A. genetic engineering. |
A technique that separates a readable pattern of DNA fragments is |
D. gel electrophoresis. |
DNA strands can be clipped crosswise at selected positions by using enzymes called |
C. restriction endonucleases. |
Geneticists can make complimentary DNA copies of messenger, transfer, and ribosomal RNA by using |
B. reverse transcriptase. |
EcoRI and HindIII are |
C. restriction endonucleases. |
Sequences of DNA that are identical when read from the 5′ to 3′ direction on one strand and the 3′ to 5′ direction on the other strand are |
A. palindromes. |
Analysis of DNA fragments in gel electrophoresis involves |
E. All of the choices are correct. |
DNA is denatured at |
D. 90 oC. |
DNA fragments can be separated in gel electrophoresis because |
D. phosphate groups have a net negative charge. |
Restriction endonucleases recognize and clip at DNA base sequences called |
B. palindromes. |
In recombinant DNA technique, what enzyme is needed to seal the sticky ends of genes into plasmids or chromosomes? |
D. DNA ligase |
Labeled, known, short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are known as |
E. gene probes. |
Two different nucleic acids can _____ by uniting at their complementary sites. |
A. hybridize |
The size of DNA is often given in the number of _____ that it contains. |
C. base pairs |
Amplification of DNA is accomplished by |
A. polymerase chain reaction. |
DNA polymerases used in PCR |
C. include Taq polymerase. |
Which PCR step causes the denaturation of double-stranded DNA? |
D. heat target DNA to 94° C |
Which PCR step synthesizes complimentary DNA strands? |
A. add DNA polymerase and nucleotides at 72° C |
The primers in PCR are |
A. synthetic DNA oligonucleotides. |
If you start with 3 double-stranded DNA fragments, after 4 cycles of PCR you will have ____ fragments. |
D. 48 |
The deliberate removal of genetic material from one organism and combining it with the genetic material of another organism is a specific technique called |
C. recombinant DNA. |
Each of the following are features of a cloning host except |
D. pathogenic. |
Common vectors used to transfer a piece of DNA into a cloning host are |
E. All of the choices are correct. |
Genomic _____ are collections of isolated genes maintained in a cloning host. |
B. libraries |
This is a promising treatment for stopping the expression of an unwanted gene: |
C. antisense therapeutic |
Choose the antisense to the following mRNA sequence: AUGAUCCGCGAUU. |
D. UACUAGGCGCUAA |
Restriction endonucleases are obtained from various species of bacteria. |
true |
When DNA is heated, the two strands will separate. |
true |
Reverse transcriptase is used to make cDNA from an RNA template. |
true |
After 3 replication cycles in PCR, there will be a total of 3 double-stranded DNA molecules. |
false |
Viruses are often used as cloning hosts in recombinant DNA methods. |
false |
Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid. |
true |
An example of gene therapy is the insertion of the gene for human growth hormone into E. coli cells. |
false |
It is now possible to very quickly map the genome of an organism or virus. |
true |
NCLEX Prep: An 18-month-old male is admitted to the hospital with symptoms of a respiratory infection. The patient’s mother reports that since birth, the toddler has frequently had a cough and copious nasal secretions. His weight gain has been poor, despite adequate oral intake. The patient appears in moderate respiratory distress, so oxygen therapy and IV fluids are initiated. The physician orders tests for cystic fibrosis due to the patient’s history and clinical presentation. When the tests confirm the Which of the following is the most appropriate term to describe the patient’s clinical manifestations of |
B. phenotype |
8 Micro – Genetics
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